ABSTRACT
Development of thoracolumbar vertebra (TLV) and rib primordium (RP) is a common evolutionary feature across vertebrates, although whole-organism analysis of the expression dynamics of TLV- and RP-related genes has been lacking. Here, we investigated the single-cell transcriptome landscape of thoracic vertebra (TV), lumbar vertebra (LV), and RP cells from a pig embryo at 27 days post-fertilization (dpf) and identified six cell types with distinct gene expression signatures. In-depth dissection of the gene expression dynamics and RNA velocity revealed a coupled process of osteogenesis and angiogenesis during TLV and RP development. Further analysis of cell type-specific and strand-specific expression uncovered the extremely high level of HOXA10 3'-UTR sequence specific to osteoblasts of LV cells, which may function as anti-HOXA10-antisense by counteracting the HOXA10-antisense effect to determine TLV transition. Thus, this work provides a valuable resource for understanding embryonic osteogenesis and angiogenesis underlying vertebrate TLV and RP development at the cell type-specific resolution, which serves as a comprehensive view on the transcriptional profile of animal embryo development.
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OBJECTIVE:To observe clinical efficacy and safety of Mailuoning injection combined with benazepril in the treat-ment of chronic glomerulonephritis. METHODS:130 patients with chronic glomerulonephritis were randomly divided into observa-tion group and control group,with 65 cases in each group. Both groups received rountine treatment as low salt and fat diet. Control group was additionally given Benazepril tablet 10 mg,qd;observation group was additionally given Mailuoning injection 20 ml added into 5% Sodium chloride injection 250 ml,ivgtt,qd,on the basis of control group,20 days of drug withdrawal every 10 days. Both group received 4 months of treatment. Clinical efficacy of 2 groups were observed as well as the levels of BUN,UCr, SCr and 24 h urine protein quantitative (UPE) before and after the treatment. The incidence of ADR was compared between 2 groups. RESULTS:Total effective rate of observation group was 83.1%,which was significantly higher than 67.7% of control group,with statistical significance (P0.05);those indexes of 2 groups decreased significantly after treatment,and the observation group were lower than the control group,with statistical significance(P0.05). CONCLUSIONS:Mailuoning injection combined with benazepril is effective for chronic glo-merulonephritis,and can effectively improve renal function with good safety.
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OBJECTIVE:To observe the clinical efficacy of Shenkang injection,a new compound preparation for chronic renal failure(CRF).METHODS:A total of 84 CRF patients were randomly divided into two groups:the treatment group(n=54)was given routine antihypertensive therapy and infused(iv gtt)with Shenkang injection 60～ 100mL(dissolved in 10% GS 300mL or NS 300mL)q.d for 30d;the control group(n=30)was infused(iv gtt)with Compound danshen injection 250mL q.d for 30d.RESULTS:As compared with the control group,serum levels of creatinine and urea nitrogen(UN)in treatment group had a greater reduction(P
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[Objective] To observe the effect of Shuhou Kangyan Decoction (SKD), a liver-kidney nourishing, Qi-strengthening and blood-activating, wind-dispelling and heat-clearing prescription mainly composed of Radix Astragali, Radix Angelicae Sinensis, Fructus Lycii, Radix Rehmanniae, Radix Saposhnikoviae, Rhizoma Atractylodis Macrocephalae, etc. , on interleukin-2 (IL-2) and soluble IL-2 receptor (SIL-2R) in serum and tears of patients with cataract after operation. [Methods] Forty-seven cases of cataract after ultrasonic emulsification plus artificial lens implantation were randomized into two groups. Group A (n = 26) was treated with routine operation and eye drops and group B ( n = 21) with SKD additionally and other 15 healthy aged people ( n = 15) receiving physical examination in the out-patient department served as normal controls. [Results] Post-operative serum SIL-2R level was higher in groups A and B than that before operation ( P 0.05). Post-operative IL-2 and SIL-2R levels in the serum and tears were higher in group A than that in groups B and C (P
ABSTRACT
Asymptomatic bacteriuria is common in diabetic patients. This article reviews several aspects of asymptomatic bacteriuria in diabetes mellitus, including prevalence, mechanisms, coexisting risk factors, bacteriology, treatment and prognosis.